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INHIBITORY EFFECT OF LACTOCOCCUS LACTIS 1370 ON THE FORMATION OF DENTAL PLAQUE IN CHILDREN

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ÀÌâ¼· ( Lee Chang-Seep ) - Á¶¼±´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç
À̱¤Èñ ( Lee Kwang-Hee ) - ¿ø±¤´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç
¿ÀÁ¾¼® ( Oh Jong-Suk ) - Àü³²´ëÇб³ ÀÇ°ú´ëÇÐ ¹Ì»ý¹°Çб³½Ç
ÀÌ»óÈ£ ( Lee Sang-Ho ) - Á¶¼±´ëÇб³ Ä¡°ú´ëÇÐ ¼Ò¾ÆÄ¡°úÇб³½Ç

Abstract

Ä¡Å ¾ïÁ¦È¿°ú°¡ ÀÖ´Â °ÍÀ¸·Î ¾Ë·ÁÁø Lactococcus lactis 1370À¸·Î Á¦Á¶ÇÑ ¾çÄ¡¿ë¾×ÀÌ ½ÇÁ¦ ¼Ò¾ÆµéÀÇ ±¸°­³»¿¡¼­ Ä¡ÅÂÀÇ Çü¼ºÀ» ¾ïÁ¦ÇÏ´ÂÁö ¿©ºÎ¿Í ÀÌµé ±ÕÁÖÀÇ ½Ã°£°æ°ú¿¡ µû¸¥ ±¸°­³» ÀÜ·ùÀ²À» ¾Ë¾Æº¸°íÀÚ ¼Ò¾Æ 30¸íÀ» ´ë»óÀ¸·Î À§¾çÄ¡¾× (Lactobacillus casei)°ú À¯»ê±Õ ¾çÄ¡¾× (Lactococcus lactis 1370)À¸·Î ¾çÄ¡Çϵµ·Ï ÇÑ µÚ ½Ã°£°æ°ú¿¡ µû¸¥ Ä¡ÅÂÁö¼ö, ġźÎÂø¸éÀûÀ», ±×¸®°í Lactococcus lactis 1370ÀÇ »ý±Õ¼ö¸¦ countÇÏ¿© ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù. 1. ´ëÁ¶±º°ú ½ÇÇ豺¿¡¼­ 24½Ã°£ÈÄ Ä¡ÅÂÁö¼ö´Â °¢°¢ Æò±Õ 2.43°ú 2.06À¸·Î ¾çÄ¡¿¡ ÀÇÇØ Ä¡ÅÂÁö¼ö´Â Æò±Õ 0.37 °¨¼ÒÇÏ¿´À¸¸ç Ä¡ÅÂÇü¼º¾ïÁ¦À²Àº ¾à 15%·Î À¯ÀÇÇÑ °¨¼Ò¸¦ º¸¿´´Ù (P<0.05). 2. 48½Ã°£ ÈÄ Ä¡ÅÂÁö¼ö´Â ´ëÁ¶±º°ú ½ÇÇ豺¿¡¼­ °¢°¢ Æò±Õ 2.95¿Í 2.17·Î ¾çÄ¡¿¡ ÀÇÇØ Ä¡ÅÂÁö¼ö´Â Æò±Õ 0.78 °¨¼ÒÇÏ¿´À¸¸ç Ä¡ÅÂÇü¼º ¾ïÁ¦À²Àº ¾à 26%·Î 24½Ã°£ÈÄ¿¡ ºñÇØ Á»´õ ¸¹Àº °¨¼Ò¸¦ º¸¿´´Ù(P<0.05). 3. ´ëÁ¶±º°ú ½ÇÇ豺¿¡¼­ 24½Ã°£ÈÄ Ä¡ÅºÎÂø¸éÀûÀ²Àº °¢°¢ Æò±Õ 21.2%¿Í 15.6%·Î ¾çÄ¡¿¡ ÀÇÇØ Ä¡ÅºÎÂø¸éÀûÀ²Àº Æò±Õ 5.63% °¨¼ÒÇÏ¿´À¸¸ç Ä¡ÅÂÇü¼º¾ïÁ¦À²Àº ¾à 26%·Î À¯ÀÇÇÑ °¨¼Ò¸¦ º¸¿´´Ù(P<0.05). 4. 48½Ã°£ÈÄ Ä¡ÅºÎÂø¸éÀûÀ²Àº ´ëÁ¶±º°ú ½ÇÇ豺¿¡¼­ °¢°¢ Æò±Õ 33%¿Í 17.8%·Î ¾çÄ¡¿¡ ÀÇÇØ Ä¡ÅºÎÂø¸éÀûÀ²Àº Æò±Õ 15.1% °¨¼ÒÇÏ¿´À¸¸ç Ä¡ÅÂÇü¼º ¾ïÁ¦À²Àº ¾à 46%·Î 24½Ã°£ ÈÄ¿¡ ºñÇØ Á» ´õ ¸¹Àº °¨¼Ò¸¦ º¸¿´´Ù(P<0.05). 5. ¾çÄ¡ÈÄ ½Ã°£¿¡ µû¸¥ ±¸°­³» »ý±Õ¼ö¸¦ countÇÑ °á°ú 1½Ã°£ ÀÌÈıîÁö´Â ±Þ°ÝÇÑ °¨¼Ò¸¦, 3½Ã°£ ÈÄ¿¡´Â ¾à°£ÀÇ °¨¼Ò¸¦ º¸¿´À¸¸ç 3½Ã°£ºÎÅÍ 6½Ã°£ »çÀÌ¿¡´Â ¾à°£ÀÇ Áõ°¡Ãß¼¼¸¦ º¸¿´´Ù.¿ä½Ã°£ÀÇ ´ÜÃà°ú ³ôÀº Á¤¹Ðµµ ¹× Á¤È®µµ·Î ¹Ýº¹ÀûÀÎ À¯±âź¼ÒºÐ¼®¿¡ À¯¿ëÇÒ °ÍÀ¸·Î »ç·áµÈ´Ù./TEX>°³¿ùÀÇ ÆíÂ÷¸¦ º¸¿´´Ù. ÀÌó·³ ¿¬·ÉÀ» ¸ð¸£´Â ¿ì¸®³ª¶óÀÇ È¥ÇÕÄ¡ ¿­±â ¾Æµ¿¿¡¼­ Æijë¶ó¸¶ ¹æ»ç¼± »çÁøÀ» ÀÌ¿ëÇÏ¿© ¿¬·É ÃßÁ¤½Ã À§ÀÇ ¹æÁ¤½ÄÀ» »ç¿ëÇÑ´Ù¸é ºñ±³Àû Á¤È®ÇÏ°Ô ¿¬·ÉÀ» ÃßÁ¤ÇÒ ¼ö ÀÖÀ¸¸®¶ó°í »ç·áµÈ´Ù. »ó¾ÆÁú Á¢ÂøÁ¦ÀÇ Á¾·ù´Â º°´Ù¸¥ ¿µÇâÀ» ÁÖÁö ¾ÊÀº °ÍÀ¸·Î ºÐ¼®µÇ¾ú´Ù. °áÇÕ°­µµÀÇ ºñ±³¿¡¼­ ÄÄÆ÷¸Ó°¡ ±Û¶ó½º ¾ÆÀ̾î³ë¸Ó¿¡ ºñÇØ ¿ì¼öÇÑ °ÍÀ¸·Î Æò°¡µÇ¾î ¼Ò¾Æ ȯÀÚÀÇ À¯±¸Ä¡ ½É¹Ì ¼öº¹Àç·á·Î¼­ÀÇ °¡´É¼ºÀÌ ÀÔÁõµÇ¾ú´Ù.ÈÄ ÁßÇÕÇÑ ±ºÀÌ »ê¼Ò¾ïÁ¦ÃþÀÇ µÎ²²°¡ Æò±Õ 49%ÀÇ °¨¼ÒµÇ¾úÀ¸¸ç(p<0.05), ÀÌµé »ê¼Ò¸¦ Â÷´ÜÇÑ ±º °£ÀÇ À¯ÀÇÂ÷´Â ¾ø¾ú´Ù.¸ç CYP1A2À¯ÀüÀÚÇü¿¡ µû¸¥ ¿µÇâÀº °üÂûÇÒ ¼ö ¾ø¾ú´Ù. CYP1A2À¯ÀüÀÚÇü¿¡ µû¸¥ »ýü³» ´ë»ç´ÉÀ» °üÂûÇÏ´Â ½ÇÇèÀÌ ÇâÈÄ ÀÌ·ç¾î Á®¾ß ÇÒ °ÍÀ¸·Î »ç·áµÈ´Ù.san filmº¸´Ù Å« ¼öÁõ±â Åõ°úµµ¸¦ º¸¿´´Ù.ÀûÀ¸·Î À¯ÀÇÇÑ Â÷À̸¦ º¸ÀÌÁö ¾Ê¾Ò´Ù.y tissue layer thinningÀº 3 ±º¸ðµÎ¿¡¼­ °üÂûµÇ¾ú°í Ç×¾Ï 3 ÀϱºÀÌ °¡Àå ½ÉÇÏ°Ô ³ªÅ¸³µ´Ù. ÀÌ»óÀÇ ½ÇÇè°á°ú¸¦ º¸¸é ¼úÀü Ç×¾ÏÁ¦Åõ¿©°¡ Ãʱ⿡ ½ÃÇàÇÑ °æ¿ì¿¡´Â Á¶Á÷ÀÇ Ä¡À¯¿¡ Ãʱâ 5 ÀÏÁ¤µµ±îÁö´Â ¿µÇâÀ» ¹ÌÄ¡³ª 7 ÀÏÀÌ Áö³ª¸é Á¤»ó¹üÁַΠȸº¹ÇÔÀ» ¾Ë¼ö ÀÖ¾ú°í ½ÇÇè°á°ú Ç×¾ÏÁ¦ Åõ¿©ÈÄ 3 ÀÏ° ÇÇÆÇ Çü¼ºÇÑ ±º¿¡¼­ ÇÇÆÇÄ¡À¯°¡ ´Ê¾îÁø °ÍÀ¸·Î °üÂûµÇ¾î ÀÎü¿¡¼­ Ç×¾Ï Åõ¿©ÈÄ ¼ö¼ú½Ã±â´Â ÀÎü¸é¿ª°è°¡ ȸº¹ÇÏ´Â ½Ã±â¸¦ 3ÁÖÀÌ»ó °æ°úÈÄ Àû¾îµµ 4ÁÖ° ¼ö¼ú½Ã±â¸¦ Á¤ÇÏ´Â °ÍÀÌ À¯¸®Çϸ®¶ó »ý°¢µÇ¾ú´Ù.ÇÑ º¹ÇÕ·¹ÁøÀº °³¹ßÀÇ Ãʱâ´Ü°èÀ̸ç, ¹°¼ºÀÇ Áõ°¡¸¦ À§ÇÑ ¿¬±¸°¡ ÇÊ¿äÇÒ °ÍÀ¸·Î »ç·áµÈ´Ù.¶Ç ´Ù¸¥ ¾à¹°ÀÎ glycyrrhetinic acid()µµ CCh ÀÚ±ØÀ¸·Î ÀÎÇÑ Å¸¾×ºÐºñ¸¦ ¾ïÁ¦ÇÏ

This study was undertaken to evaluate the clinical effect of inhibiting plaque formation of Lactococcus lactis 1370, a acid producing bacterium residing in the mouth. 30 children were asked to use 10ml of control mouth-wash and mouthwash containing Lactococcus lactis 1370. The plaque index and plaque area rate at 24h and 48h after the use of the mouthwashes were measured. And the number of Lactococcus lactis 1370 was counted at 1h, 3h, and 6h in the mouth. The results are as follow. 1. The mean plaque index at 24h after the use of the control mouthwash and the mouthwash containing Lactococcus lactis 1370 were 2.43 and 2.06, respectively. The inhibiting rate of plaque formation was 15% (P<0.05). 2. The mean plaque index at 48h after the use of the control mouthwash and the mouthwash containing Lactococcus lactis 1370 were 2.95 and 2.17, respectively. The inhibiting rate of plaque formation was 26%, showing more decrease than at 24h(P<0.05). 3. The mean plaque area rate at 24h after the use of the control mouthwash and the mouthwash containing Lactococcus lactis 1370 were 21.2% and 15.6%, respectively. The inhibiting rate of plaque formation was 26% (P<0.05). 4. The mean plaque area rate at 48h after the use of the control mouthwash and the mouthwash containing Lactococcus lactis 1370 were 33.0% and 17.8%, respectively. The inhibiting rate of plaque formation was 46% (P<0.05). 5. The number of Lactococcus lactis 1370 in the mouth decreased significantly from mouthwashing to 3h, but increased slightly between 3h and 6h. As seen with the above results, we think that using the mouth wash with Lactococcus lactis 1370 would prevent the formation of plaque in the mouth and can be an effective method to prevent dental caries and periodontal disease.

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Mouthwash;Lactococcus lactis;Dental caries;Plaque index

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